mouse: icr  (clea japan inc)

Journal: Scientific Reports

Article Title: Aluminum adjuvants differentially induce IL-1β release in vitro yet share NLRP3 inflammasome-independent adjuvant effects in vivo

doi: 10.1038/s41598-025-34660-6

Figure Lengend Snippet: Dose-dependent inflammasome activation and cytotoxicity in LPS-primed mouse macrophages and dendritic cells stimulated with AH or AP. BMDMs ( a–c ) and BMDCs ( d – f ) derived from CD-1 mice were incubated in medium only (M) or primed with LPS and stimulated with increasing concentrations of AH or AP for 24 h. ( a , d ). Cytotoxicity was assessed by measuring lactate dehydrogenase (LDH) release. ( b , e ) IL-1β secretion was quantified by ELISA. ( c , f ) Western blot analysis for IL-1β of supernatants (Sup) and lysates (Lys). Ten µg of total protein was loaded per lane for BMDMs (c) and 20 µg for BMDCs (f). Bars represent the mean ± SEM of triplicate wells. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).

Article Snippet: Female Hsd:ICR (CD-1®) (CD-1) mice were purchased from Inotiv (Indianapolis, IN).

Techniques: Activation Assay, Derivative Assay, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Scientific Reports

Article Title: Aluminum adjuvants differentially induce IL-1β release in vitro yet share NLRP3 inflammasome-independent adjuvant effects in vivo

doi: 10.1038/s41598-025-34660-6

Figure Lengend Snippet: The antibody response to protein antigens injected with aluminum adjuvants does not require functional NLRP3. ( A , B ) C57BL/6J WT and Nlrp3 -/- mice were injected twice with OVA and AH ( A ) or nucleoprotein of SARS-CoV-2 (NP) and AP ( B ). The graphs show the mean ± SE of four mice per group. ( C , D ) CD-1 mice were treated with MCC950 or untreated and then injected twice with OVA and AH ( C ) or nucleoprotein of SARS-CoV-2 (NP) and AP ( D ). The graphs show data from individual mice and the mean ± SE of 4–5 mice per group. ns – not significant (Student’s t-test, p > 0.05).

Article Snippet: Female Hsd:ICR (CD-1®) (CD-1) mice were purchased from Inotiv (Indianapolis, IN).

Techniques: Injection, Functional Assay

Journal: Journal of Biomedical Science

Article Title: Development of mRNA–lipid nanoparticle intrabodies against rickettsial infection

doi: 10.1186/s12929-025-01171-5

Figure Lengend Snippet: A44 and A123 mRNA-LNPs reduce E. chaffeensis infection in HEK293 cells and mice. A - B HEK293 cells were incubated with LNP-mRNA-IBs or PBS control (CTL) for 6 h and infected with E. chaffeensis for 30 h. (A) Ehrlichia infection was determined by RT-qPCR analysis using primers specific for E. chaffeensis 16S rRNA gene normalized to human ACTB . Data are presented as the mean ± SD from three independent experiments. *Significantly different as determined by one-way ANOVA followed by Dunnett’s test (CTL vs A44, P = 0.0024; CTL vs A123, P = 0.0008; CTL vs A44 + A123, P = 0.0004). B WB showing expression of IBs using antibodies against HA-tagged IBs and human Actin. C - E ICR mice were inoculated intraperitoneally with E. chaffeensis –infected THP-1 cells that had been preincubated with an mRNA-LNP (A44, A123, or A171) or PBS (CTL) for 12 h. At 1 and 3 d after inoculation, mice were injected intravenously with the same mRNA-LNP or PBS. All mice were euthanized at 5 dpi to collect blood, liver, and spleen samples. C Infection with Ehrlichia was determined by qPCR of blood samples using primers specific to the E. chaffeensis ( Ech ) 16S rRNA gene. Results were normalized based on mouse Gapdh expression. D - E Ehrlichia load in spleen (D) and liver (E) samples was estimated by RT-qPCR as in ( A ). The scatter plots show normalized levels in individual mice, with the horizontal bar representing the mean value ( n = 5). * Significantly different as determined by one-way ANOVA followed by Dunnett’s test with data from blood (CTL vs A44, P = 0.0479; CTL vs A123, P = 0.0318), spleen (CTL vs A44, P = 0.001; CTL vs A123, P = 0.0024), and liver (CTL vs A44, P = 0.0322; CTL vs A123, P = 0.0192)

Article Snippet: To deliver IB mRNA-LNPs in vivo and to examine their effects on Ehrlichia infection, ICR mice (five mice per group; Inotiv, West Lafayette, IN) were intraperitoneally inoculated with E. chaffeensis cultured in THP-1 cells (~ 2 × 10 5 bacteria per mouse), which were preincubated with IB mRNA-LNPs (A44, A123, or A171) or with PBS for 12 h. As E. chaffeensis infects blood monocytes and macrophages, intravenous injection of LNP-mRNAs allows immediate delivery to the bloodstream and rapid systemic distribution to infected cells [ ].

Techniques: Infection, Incubation, Control, Quantitative RT-PCR, Expressing, Injection